Traditional Glycan Methods

The traditional method of glycan analysis is laborious, and
involves many steps, starting with enzymatic glycan release
by PNGaseF (overnight), followed by sample cleanup,
labeling with a fluorescence tag by reductive amination (2-AB
or InstantPC), and finally cleanup of the released labeled
N-glycans prior to LC-FLD or LC/MS analysis2,3. Despite the
significant improvement of MS sensitivity using fluorescent
tags, the labor intensiveness of manual sample preparation,
low reproducibility, and limitation to scale-up on sample
processing have been major issues for the biopharmaceutical
industry.